Phenotypic Detection of β-Lactamase Resistance in Gram-Negative Bacilli

To detect isolates with AmpC, K1, and KPC enzymes, as well as those with ESBLs, it is desirable to test all Enterobacteriaceae with a combination of antibiotics that will allow detection of resistant mechanisms. In Dr. Paul Schreckenberger's laboratory, he and Violeta Rekasius, MT(ASCP) have expanded the ESBL confirmatory disk test to include 12 antibiotic disks and have applied the test to all members of the Enterobacteriaceae that have a susceptibility pattern that is suspicious for the presence of an ESBL, AmpC, K1 or KPC type resistance gene. Through the application of this test they have been able to successfully detect antibiotic resistance in many species of Enterobacteriaceae that would have not been detected using their automated susceptibility testing system.

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