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Figure 1: Bacillus cereus_acid fast (Enlarged view). FIG. 1. Bacillus cereus, non-acid-fast. (Jerry Keplinger, East Tennessee State University, Johnson City, TN)

Figure 2: Bacillus cereus_acid fast (Enlarged view). FIG. 2. Bacillus cereus, non-acid-fast. (Jerry Keplinger, East Tennessee State University, Johnson City, TN)

Figure 3: Mycobacterium smegmatis_acid fast (Enlarged view). FIG. 3. Mycobacterium smegmatis acid-fast stain using the Kinyoun acid-fast procedure. (Jerry Keplinger, East Tennessee State University, Johnson City, TN)

Figure 4: Mycobacterium smegmatis_acid fast (Enlarged view). FIG. 4. Mycobacterium smegmatis acid-fast stain using the Kinyoun acid-fast procedure. (Jerry Keplinger, East Tennessee State University, Johnson City, TN)

Figure 5: Mycobacterium smegmatis_acid fast (Enlarged view). FIG. 5. Mycobacterium smegmatis acid-fast stain using the Kinyoun acid-fast procedure. (Jerry Keplinger, East Tennessee State University, Johnson City, TN)

Figure 6: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 6. Mycobacterium tuberculosis from Mycobacteria growth indicator tube. Note Kinyoun cording where the mycobacterial acid-fast-positive rods grow together in "cords" or ropes. (Sarah Zimmerman, Lahey Clinic, Burlington, MA)

Figure 7: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 7. Mycobacterium tuberculosis from Mycobacteria growth indicator tube. Note Kinyoun cording where the mycobacterial acid-fast-positive rods grow together in "cords" or ropes. Samples were viewed at 400x magnification. (Sarah Zimmerman, Lahey Clinic, Burlington, MA)

Figure 8: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 8. Mycobacterium tuberculosis from Mycobacteria growth indicator tube. Note Kinyoun cording where the mycobacterial acid-fast-positive rods grow together in "cords" or ropes. (Sarah Zimmerman, Lahey Clinic, Burlington, MA)

Figure 9: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 9. Mycobacterium tuberculosis strain H37Rv showing the in vivo M. tuberculosis isolated from guinea pig lungs after 90-day aerosol infection. (Libin Shi and Delphi Chatterjee, Colorado State University, Fort Collins, CO)

Figure 10: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 10. Mycobacterium tuberculosis strain H37Rv showing the in vivo M. tuberculosis isolated from guinea pig lungs after 90-day aerosol infection. (Libin Shi and Delphi Chatterjee, Colorado State University, Fort Collins, CO)

Figure 11: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 11. Mycobacterium tuberculosis strain H37Rv showing the in vitro cultured M. tuberculosis H37Rv. (Libin Shi and Delphi Chatterjee, Colorado State University, Fort Collins, CO)

Figure 12: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 12. Mycobacterium tuberculosis strain H37Rv showing the in vitro cultured M. tuberculosis H37Rv. (Libin Shi and Delphi Chatterjee, Colorado State University, Fort Collins, CO)

Figure 13: Mycobacterium smegmatus and Staphylococcus epidermidis_acid fast (Enlarged view). FIG. 13. A mixed culture of Mycobacterium smegmatus (acid-fast) and Staphylococcus epidermidis (non-acid-fast). (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 14: Mycobacterium smegmatus and Staphylococcus epidermidis_acid fast (Enlarged view). FIG. 14. A mixed culture of Mycobacterium smegmatus (acid-fast) and Staphylococcus epidermidis (non-acid-fast). (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 15: Mycobacterium smegmatus and Staphylococcus epidermidis_acid fast (Enlarged view). FIG. 15. A mixed culture of Mycobacterium smegmatus (acid-fast) and Staphylococcus epidermidis (non-acid-fast). (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 16: Mycobacterium smegmatus and Staphylococcus epidermidis_acid fast (Enlarged view). FIG. 16. A mixed culture of Mycobacterium smegmatus (acid-fast) and Staphylococcus epidermidis (non-acid-fast). (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 17: Mycobacterium smegmatus and Staphylococcus epidermidis_acid fast (Enlarged view). FIG. 17. A mixed culture of Mycobacterium smegmatus (acid-fast) and Staphylococcus epidermidis (non-acid-fast). (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 18: Mesenteric lymph node_acid fast (Enlarged view). FIG. 18. Mesenteric lymph node from a bison with naturally occurring Johne's disease (Mycobacterium avium subsp. paratuberculosis). (Debra Kamstock, Colorado State University, Fort Collins, CO)

Figure 19: Mesenteric lymph node_acid fast (Enlarged view). FIG. 19. Mesenteric lymph node from a bison with naturally occurring Johne's disease (Mycobacterium avium subsp. paratuberculosis). (Debra Kamstock, Colorado State University, Fort Collins, CO)

Figure 20: Mesenteric lymph node_acid fast (Enlarged view). FIG. 20. Mesenteric lymph node from a bison with naturally occurring Johne's disease (Mycobacterium avium subsp. paratuberculosis). (Debra Kamstock, Colorado State University, Fort Collins, CO)

Figure 21: Mesenteric lymph node_acid fast (Enlarged view). FIG. 21. Mesenteric lymph node from a bison with naturally occurring Johne's disease (Mycobacterium avium subsp. paratuberculosis). (Debra Kamstock, Colorado State University, Fort Collins, CO)

Figure 22: Mesenteric lymph node_acid fast (Enlarged view). FIG. 22. Mesenteric lymph node from a bison with naturally occurring Johne's disease (Mycobacterium avium subsp. paratuberculosis). (Debra Kamstock, Colorado State University, Fort Collins, CO)

Figure 23: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 23. Human peripheral blood monocyte derived macrophages (HPBMDM) with intracellular acid-fast stained Mycobacterium tuberculosis. After 5 days of culture of HPBMDM (1.0 x 106 cells/ml) in RPMI medium containing 2 mM L-glutamine and autologous human serum, approximately 1.0 x 105 M. tuberculosis cells in 0.010 ml (which had previously been found to result in optimum phagocytosis) were added to monolayer cultures of monocyte-derived macrophages. The slides are stained for acid-fast bacilli and counterstained with Meyer's hematoxylin and thereafter mounted with crystal mount. Panel A shows a large macrophage (blue) with intracellular acid-fast-staining bacilli (red, arrow) and panel B shows a macrophage with intracellular acid-fast-staining bacilli (red, arrow) and round shaped lymphocytes (blue). Bars, 10 µm. (Diane Ordway Rodriguez, Randall Basaraba, and Ian Orme, Colorado State University, Fort Collins, CO)

Figure 24: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 24. Murine lung tissue aveolar macrophages with intracellular acid-fast-stained Mycobacterium tuberculosis. Representative photomicrographs are taken from mice exposed to a low dose aerosol of M. tuberculosis. Lung sections are stained for acid-fast bacilli and counterstained with Meyer's hematoxylin and thereafter mounted with crystal mount. Panels A and B demonstrated a large number of highly vacuolated foamy macrophages (arrows) containing a large number of acid-fast-positive-staining bacilli (red, arrow). Panel B shows M. tuberculosis in large aggregates (bold arrow) and single bacilli (arrow). Bars: A, 100 µm and B, 10 µm for the high-power image. (Diane Ordway Rodriguez, Randall Basaraba, and Ian Orme, Colorado State University, Fort Collins, CO)

Figure 25: Mycobacterium tuberculosis_acid fast (Enlarged view). FIG. 25. Guinea pig lung tissue aveolar macrophages with intracellular acid-fast-stained Mycobacterium tuberculosis. Representative photomicrographs are taken from guinea pigs exposed to a low dose aerosol of M. tuberculosis. Lung sections are stained for acid-fast bacilli and counterstained with Meyer's hematoxylin and thereafter mounted with crystal mount. Panels A and B demonstrated macrophages (arrows) containing acid-fast-positive-staining bacilli (red, arrows). Panel B shows single M. tuberculosis bacilli (arrows). Bars: A, 100 µm and B, 10 µm for the high-power image. (Diane Ordway Rodriguez, Randall Basaraba, and Ian Orme, Colorado State University, Fort Collins, CO)

Figure 26: Acid-fast stain of Mycobacterium smegmatis and Staphylococcus aureus. FIG. 26. Acid-fast stain of Mycobacterium smegmatis and Staphylococcus aureus. Mixed culture smear stained according to acid-fast stain protocol; red- and pink-staining bacilli are acid-fast while blue-staining cocci are on-acid-fast.  (Tasha Sturm, Cabrillo College, Aptos, CA)