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Figure 1: Indole test results with sulfur-indole-motility (SIM) agar. SIM medium is a bacterial growth medium that is designed to test for multiple characteristics in an effort to identify an organism. All tubes were incubated for 48 hours at 37.

Figure 2: Results of the indole test using sulfur-indole-motility medium. The positive indole result given by Escherichia coli (right) is indicated by the red layer at the top of the tube after the addition of Kov

Figure 3: Result of the indole test using sulfur-indole-motility medium and Proteus vulgaris after a 24-hour incubation at 37

Figure 4: Results of the indole test using sulfur-indole-motility medium after a 24-hour incubation at 37

Figure 5: Result of the indole test using sulfur-indole-motility medium and Escherichia coli after a 24-hour incubation at 37

Figure 6: Reactions on sulfide-indole-motility (SIM) medium. Tube 1: Hydrogen sulfide production. Tube 2: Indole production. Tube 3: Motility. Tube 4: Nonmotile organism. (Anh-Hue Tu, Georgia Southwestern State University, Americus, GA)

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