Figure 1: This image shows growing clusters of Batrachochytrium dendrobatidis grown at 24°C on 1% tryptone agar for seven days. On solid media, the mature organism appears as small vanilla-colored colonies ranging in size from 1mm to 5mm in diameter. Each colony visible on the plate arose when one or more zoospores encysted in the media and grew into multinucleated, fertile sporangia (1,5). A sporangium, by definition, is a structure that produces and contains asexual spores. The ideal temperature range in which B. dendrobatidis grows is between 17°C and 25°C (6).
Figure 2: This image shows the fungal morphology of Batrachochytrium dendrobatidis at 1000X magnification using bright field imaging. The specimen was stained using Lactophenol-Cotton Blue Stain (VWR) and reveals a mature, asexual sporangium with its associated zoospores and discharge papillae.
Figure 3: Batrachochytrium dendrobatidis, stained with Lactophenol-Cotton Blue and viewed at 1000X Magnification.
Figure 4: Batrachochytrium dendrobatidis, stained with Lactophenol-Cotton Blue and viewed at 1000X Magnification (Labeled view).
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