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Authors: Emmanuel Brako, Linda Phaire-Washington​

Citation: Emmanuel Brako, Linda Phaire-Washington. 2009. Cytopathic effects of viruses: group 4 unstained infected cell lines other than bovine fetal spleen cells.​

Publication Date: December 2009​

FIG. 1. Unstained neuroblastoma (SK-N-SH) cell monolayer 72 hours postinfection at low multiplicity of infection (MOI) with equine herpesvirus type 1 showing cell rounding and retraction from the substrate leaving gaps in the monolayer. Cells were observed using phase-contrast microscopy at 128X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)​

FIG. 2. Unstained rat lung fibroblast monolayer 72 hours postinfection at low MOI with equine herpesvirus type 1 showing cell destruction as characterized by cell rounding and retraction from the substrate leaving gaps in the monolayer. Cells were observed using phase-contrast microscopy at 128X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)​

FIG. 3. Unstained rabbit kidney (LLC-RK1) cell monolayer 6 hours postinfection at low MOI with equine herpesvirus type 1 showing a focal area of polykaryocyte (also called syncytium or giant cell) (green arrow). Cells were observed using phase-contrast microscopy at 320X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)​

FIG. 4. Unstained rabbit kidney (LLC-RK1) cell monolayer 24 hours postinfection at low MOI with equine herpesvirus type 1 showing a focal area of polykaryocyte (also called syncytium or giant cell). Cells were observed using phase-contrast microscopy at 320X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)​