PowerPoint Contents
Figure 1: Spore stain_Endospore stain (Enlarged view). FIG. 1. Spore stain(Jerry Keplinger, East Tennessee State University, Johnson City, TN)
Figure 2: Bacillus sp._Endospore stain (Enlarged view). FIG. 2. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 3: Bacillus sp._Endospore stain (Enlarged view). FIG. 3. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 4: Bacillus sp._Endospore stain (Enlarged view). FIG. 4. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 5: Bacillus sp._Endospore stain (Enlarged view). FIG. 5. Endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 6: Bacillus subtilis_Endospore stain (Enlarged view). FIG. 6. Endospore-positive Bacillus subtilis. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 7: Staphylococcus sp._Endospore stain (Enlarged view). FIG. 7. Endospore-negative Staphylococcus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 8: Staphylococcus sp._Endospore stain (Enlarged view ). FIG. 8. Endospore-negative Staphylococcus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 9: Bacillus sp._Endospore stain (Enlarged view). FIG. 9. Phase-contrast image of endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 10: Bacillus sp._Endospore stain (Enlarged view). FIG. 10. Phase-contrast image of endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 11: Bacillus sp._Endospore stain (Enlarged view). FIG. 11. Phase-contrast image of endospore-positive Bacillus species. (Ann C. Smith, University of Maryland, College Park, MD)
Figure 12: Bacillus subtilis_Endospore stain (Enlarged view). FIG. 12. Bacillus subtilis, an endospore-producing bacterium. (Brian Kimble, University of Maryland, College Park, MD)
Figure 13: Bacillus cereus_Endospore stain (Enlarged view). FIG. 13. Endospore stain of a Bacillus cereus culture using the Shaeffer-Fulton method and viewed at 1,000x total magnification under an oil immersion lens. Vegetative cells of B. cereus are in red; endospores are in green. Due to the age of the culture, endospores have been released from the cells. (Derek Weber and S. Finazzo, Broward Community College–Central campus, Davie, FL)
Figure 14: Bacillus sp._Endospore stain (Labeled view). FIG. 14. Bacillus spp. stained with Sheaffer-Fulton technique showing green endospores. (Javier Gutierrez Jimenez, Sciences and Arts University of Chiapas, Mexico)
Figure 14: Bacillus sp._Endospore stain (Enlarged view). FIG. 14. Bacillus spp. stained with Sheaffer-Fulton technique showing green endospores. (Javier Gutierrez Jimenez, Sciences and Arts University of Chiapas, Mexico)
Figure 15: Bacillus subtilis_Endospore stain (Enlarged view). FIG. 15. Bacillus subtilis. (Alan Schenkel, Peter Justice and Erica Suchman, Colorado State University, Fort Collins, CO)
Figure 16: Bacillus subtilis_Endospore stain (Enlarged view). FIG. 16. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
Figure 17: Bacillus subtilis _Endospore stain (Enlarged view). FIG. 17. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
Figure 18: Bacillus subtilis _Endospore stain (Enlarged view). FIG. 18. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
Figure 19: Bacillus subtilis _Endospore stain (Enlarged view). FIG. 19. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
Figure 20: Bacillus subtilis _Endospore stain (Enlarged view). FIG. 20. Bacillus subtilis. (Alan Schenkel, Peter Justice, and Erica Suchman, Colorado State University, Fort Collins, CO)
Figure 21: Staining of Bacillus megaterium endospores. FIG. 21. Staining of Bacillus megaterium endospores using the Schaeffer-Fulton method. Bacteria such as Bacillus megaterium form endospores when environmental conditions become unfavorable for growth. Endospores are metabolically inactive and can remain dormant for many years until favorable conditions return. The resting endospores are highly resistant to many factors such as heat, chemicals, dryness, and radiation. They are not easily destroyed and are difficult to stain. In this method, a slide containing a heat-fixed smear of Bacillus megaterium was steam treated to allow the malachite green to penetrate the spore coat. The vegetative cells were counterstained using safranin. As a result, the endospores appeared green and the vegetative cells appeared red. For more details of the staining procedure, see the references listed below. (Anh-Hue T. Tu, Georgia Southwestern State University, Americus, GA)
Figure 22: Staining of Bacillus megaterium endospores (Labeled view). FIG. 22. Staining of Bacillus megaterium endospores using the Schaeffer-Fulton method. Labeled view. (Anh-Hue T. Tu, Georgia Southwestern State University, Americus, GA)
Figure 23: Endospore stain of Bacillus cereus. FIG. 23. Endospore stain of Bacillus cereus. Vegetative cells (red rods) were stained according to the endospore stain protocol and produced the green-staining endospores. (Tasha Sturm, Cabrillo College, Aptos, CA)
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