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Figure 1: Uninoculated Phenylethyl Alcohol Agar Plate. An uninoculated phenylethyl alcohol (PEA) agar plate with 5% sheep blood. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 2: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Staphylococcus aureus. Phenylethyl alcohol (PEA) agar with 5% sheep blood inoculated with Staphylococcus aureus, a gram-positive bacterium, showing good growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 3: Phenylethyl Alcohol Agar Plate with 5% Sheep Blood Inoculated with Streptococcus pyogenesPhenylethyl alcohol (PEA) agar plate with 5% sheep blood inoculated with Streptococcus pyogenes, a gram-positive bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 4: Phenylethyl Alcohol Agar Plate with 5% Sheep Blood Inoculated with Streptococcus pnuemoniae. Phenylethyl alcohol (PEA) agar plate with 5% sheep blood inoculated with Streptococcus pneumoniae, a gram-positive bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 5: Phenytlethyl Alcohol Agar Plate with 5% Sheep Blood Inoculated with Bacillus subtilis. Phenylethyl alcohol (PEA) agar plate with 5% sheep blood inoculated with Bacillus subtilis, a gram-positive bacterium, showing good growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 6: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Clostridium perfringensPhenylethyl alcohol (PEA) with 5% sheep blood inoculated with Clostridium perfringens, a gram-positive bacterium, showing partial growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 7: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Escherichia coli. Phenylethyl alcohol (PEA) agar with 5% sheep blood inoculated with Escherichia coli, a gram-negative coliform bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 8: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Salmonella enteritidis. Phenylethyl alcohol (PEA) agar with 5% sheep blood inoculated with Salmonella enteritidis, a gram-negative coliform bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 9: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Enterobacter aerogenes. Phenylethyl alcohol (PEA) agar with 5% sheep blood inoculated with Enterobacter aerogenes, a gram-negative coliform bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 10: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Pseudomonas aeruginosa. Phenylethyl alcohol (PEA) agar with 5% sheep blood inoculated with Pseudomonas aeruginosa, a gram-negative bacterium, showing partially inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 11: Phenylethyl Alcohol Agar with 5% Sheep Blood Inoculated with Proteus mirabilis. Phenylethyl alcohol (PEA) agar with 5% sheep blood inoculated with Proteus mirabilis, a gram-negative coliform bacterium, showing partially inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 12: Uninoculated Phenylethyl Alcohol Agar Plate. An uninoculated phenylethyl alcohol (PEA) agar plate. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 13: Phenylethyl Alcohol Agar Inoculated with Staphylococcus aureus. Phenylethyl alcohol (PEA) agar plate inoculated with Staphylococcus aureus, a gram-positive bacterium, showing good growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 14: Phenylethyl Alcohol Agar Inoculated with Streptococcus pyogenes. Phenylethyl alcohol (PEA) agar plate inoculated with Streptococcus pyogenes, a gram-positive bacterium, showing growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 15: Phenylethyl Alcohol Agar Inoculated with Streptococcus pneumoniae. Phenylethyl alcohol (PEA) agar plate inoculated with Streptococcus pneumoniae, a gram-positive bacterium, showing growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 16: Phenylethyl Alcohol Agar Inoculated with Bacillus subtilis. Phenylethyl alcohol (PEA) agar plate inoculated with Bacillus subtilis, a gram-positive bacterium, showing good growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 17: Phenylethyl Alcohol Agar Inoculated with Clostridium perfringens. Phenylethyl alcohol (PEA) agar plate inoculated with Clostridium perfringens, a gram-positive bacterium, showing limited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 18: Phenylethyl Alcohol Agar Inoculated with Enterococcus faecalis. Phenylethyl alcohol (PEA) agar plate inoculated with Enterococcus faecalis, a gram-positive coliform bacterium, showing good growth. (Archana Lal, Independence Community College, Independence, KS)

Figure 19: Phenylethyl Alcohol Agar Inoculated with Micrococcus luteus. Phenylethyl alcohol (PEA) agar plate inoculated with Micrococcus luteus, a gram-positive coliform bacterium, showing good growth. (Archana Lal, Independence Community College, Independence, KS)

Figure 20: Phenylethyl Alcohol Agar Inoculated with Escherichia coli. Phenylethyl alcohol (PEA) agar plate inoculated with Escherichia coli, a gram-negative coliform bacterium, showing no growth. (Archana Lal, Independence Community College, Independence, KS)

Figure 21: Phenylethyl Alcohol Agar Inoculated with Salmonella enteritidis. Phenylethyl alcohol (PEA) agar plate inoculated with Salmonella enteritidis, a gram-negative coliform bacterium, showing no growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 22: Phenylethyl Alcohol Agar Inoculated with Enterobacter aerogenes. Phenylethyl alcohol (PEA) agar plate inoculated with Enterobacter aerogenes, a gram-negative coliform bacterium, showing inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 23: Phenylethyl Alcohol Agar Inoculated with Psuedomonas aeruginosa. Phenylethyl alcohol (PEA) agar plate inoculated with Pseudomonas aeruginosa, a gram-negative bacterium, showing inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 24: Phenylethyl Alcohol Agar Inoculated with Proteus mirabilis. Phenylethyl alcohol (PEA) agar plate inoculated with Proteus mirabilis, a gram-negative coliform bacterium, showing inhibited growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 25: Phenylethyl Alcohol Agar Inoculated with Morexella catarrhalis. Phenylethyl alcohol (PEA) agar plate inoculated with Morexella catarrhalis, a gram-negative bacterium, showing no growth. (Archana Lal, Independence Community College, Independence, KS)

Figure 26: Phenylethyl Alcohol Agar Inoculated with a Mixture of Gram-Positive and Gram-Negative Bacteria. A phenylethyl alcohol (PEA) agar plate inoculated with a mixture of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.  Only growth of the gram-positive bacteria is seen on the PEA agar.  When inoculated on tryptic soy agar plates, both bacterial types show growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 27: Tryptic Soy Agar Inoculated with a Mixture of Gram-Positive and Gram-Negative Bacteria Tryptic soy agar (TSA) plate inoculated with a mixture of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.  Growth of both bacterial types is seen on the TSA plate. When this mixture is inoculated on a phenylethyl alcohol (PEA) agar plate, only the gram-positive bacteria shows growth. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, British Columbia, Canada)

Figure 28: Phenylethyl Alcohol Agar Plate. Phenylethyl alcohol (PEA) agar plate inoculated with (a) gram-positive Bacillus subtilis, (b) gram-positive Micrococcus luteus, (c) gram-positive Staphylococcus aureus and (d) gram-negative Escherichia coli. The gram-positive bacteria all show good growth while the gram-negative bacterium shows no growth. (Archana Lal, Independence Community College, Independence, KS)

Figure 29: Uninoculated Phenylethyl Alcohol Agar Plate. An uninoculated phenylethyl alcohol (PEA) agar plate. (Archana Lal, Independence Community College, Independence, KS)

Figure 30: Infected Human Urine Sample Plated on Sheep Blood Agar and Phenylethyl Alcohol Blood Agar. Infected human urine sample plated on sheep blood agar and phenylethyl alcohol (PEA) blood agar illustrating the power of PEA to separate a gram-positive organism (Staphylococcus saprophyticus) from a gram-negative organism (Escherichia coli). (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Figure 31: Infected Human Urine Sample Plated on Sheep Blood Agar and Phenylethyl Alcohol Blood Agar (Labeled view). Infected human urine sample plated on sheep blood agar and phenylethyl alcohol (PEA) blood agar illustrating the power of PEA to separate a gram-positive organism (Staphylococcus saprophyticus) from a gram-negative organism (Escherichia coli). (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Figure 32: Staphylococcus saprophyticus from Infected Human Urine Growing on Phenylethyl Alcohol Agar. Staphylococcus saprophyticus from infected human urine growing on phenylethyl alcohol (PEA) agar.  The Escherichia coli known to be present in this sample failed to grow on PEA. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Figure 33: Staphylococcus saprophyticus from Infected Human Urine Growing on Phenylethyl Alcohol Agar (Labeled view). Staphylococcus saprophyticus from infected human urine growing on phenylethyl alcohol (PEA) agar. The Escherichia coli known to be present in this sample failed to grow on PEA. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Figure 34: Sheep Blood Agar Inoculated with Infected Human Urine. Sheep blood agar inoculated with infected human urine showing the growth of two bacterial species, Escherichia coli and Staphylococcus saprophyticus. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Figure 35: Sheep Blood Agar Inoculated with Infected Human Urine (Labeled view). Sheep blood agar inoculated with infected human urine showing growth of two bacterial species, Escherichia coli and Staphylococcus saprophyticus. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Figure 36: Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis Streaked on Phenylethyl Alcohol Agar. Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis streaked on phenylethyl alcohol (PEA) agar. PEA agar is selective for gram-positive organisms, particularly gram-positive cocci such as: (A) Micrococcus luteus and (B) Staphylococcus epidermidis, which exhibit good growth on the medium. The growth of (C) Klebsiella pneumoniae, a gram-negative organism, is inhibited by the presence of phenylethyl alcohol because phenylethyl alcohol is bacteriostatic for gram-negative bacteria. (Kim R. Finer, Kent State University at Stark, N. Canton, OH)

Figure 37: Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis Streaked on Phenylethyl Alcohol Agar (Labeled view). Klebsiella pneumoniae, Micrococcus luteus, and Staphylococcus epidermidis streaked on phenylethyl alcohol (PEA) agar. PEA agar is selective for gram-positive organisms, particularly gram-positive cocci such as: (A) Micrococcus luteus and (B) Staphylococcus epidermidis, which exhibit good growth on the medium. The growth of (C) Klebsiella pneumoniae, a gram-negative organism, is inhibited by the presence of phenylethyl alcohol because phenylethyl alcohol is bacteriostatic for gram-negative bacteria. (Labeled view) (Kim R. Finer, Kent State University at Stark, N. Canton, OH)

Figure 38: Phenylethyl Agar. Bacterial growth on nutrient agar versus phenylethyl alcohol (PEA) agar. Escherichia coli (left side of plate) and Staphylococcus epidermidis (right side of plate) were plated on nutrient agar (left plate) and PEA agar (right plate). Both plates were incubated at 37°C for 24 hours. On nutrient agar, both cultures grew well. On PEA agar, however, growth of the gram-negative bacterium E. coli was inhibited, whereas the gram-positive bacterium S. epidermidis flourished. (Clarissa L. Kaup and J. L. Henriksen, Bellevue University, Bellevue, NE)

Figure 39: Phenylethyl Alcohol Agar. Bacterial growth on phenylethyl alcohol (PEA) agar. Escherichia coli (left side of plate) and Staphylococcus epidermidis (right side of plate) were plated on PEA agar and incubated at 37°C for 24 hours. Growth of the gram-negative bacterium E. coli was inhibited, whereas the gram-positive organism S. epidermidis grew without inhibition. (Clarissa L. Kaup and J. L. Henriksen, Bellevue University, Bellevue, NE)

Figure 40: Phenylethyl Alcohol Agar. Bacterial growth on phenylethyl alcohol (PEA) agar. Escherichia coli (left side of plate) and Staphylococcus epidermidis (right side of plate) were plated on PEA agar and incubated at 37°C for 24 hours. Growth of the gram-negative bacterium E. coli was inhibited, whereas the gram-positive organism S. epidermidis grew without inhibition. (Clarissa L. Kaup and J. L. Henriksen, Bellevue University, Bellevue, NE)