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Figure 1: Streptomycetes Sabouraud (Enlarged view). FIG. 1. Streptomycetes and molds. This image shows molds (pink and green because of the color of the conidia) plus small, chalky white (sporulating), black or brown colonies that are streptomycetes. (Begoña Alonso-Urmeneta and Ignacio Moriyón, University of Navarra, Spain)

Figure 2: Streptomycetes Sabouraud (Enlarged view). FIG. 2. Streptomycetes and molds. Some of the brown streptomycetes make a diffusible pigment. A glossy colony in the upper right corner is a typical bacterial colony. Note the small chalky streptomycete colony in the center. (Begoña Alonso-Urmeneta and Ignacio Moriyón, University of Navarra, Spain)

Figure 3: Streptomycetes Sabouraud (Enlarged view). FIG. 3. Streptomycetes and molds. This image shows the edge of a bacterial colony in the center, and the mycelial growth of a mold (lower part) and of a streptomycete (upper part). (Begoña Alonso-Urmeneta and Ignacio Moriyón, University of Navarra, Spain)

Figure 4: Leveduras Sabouraud (Enlarged view). FIG. 4. Leveduras on Sabouraud agar. (Jane Mary Lafayette Neves Gelinski, Universidade do Oeste de Santa Catarina, Brazil)

Figure 5: Penicillium glabrum Sabouraud (Enlarged view). FIG. 5. Penicillium glabrum on Sabouraud agar. (Jane Mary Lafayette Neves Gelinski, Universidade do Oeste de Santa Catarina, Brazil)

Figure 6: Penicillium roqueforti Sabouraud (Enlarged view). FIG. 6. Penicillium roqueforti on Sabouraud agar. (Jane Mary Lafayette Neves Gelinski, Universidade do Oeste de Santa Catarina, Brazil)

Figure 7: Saccharomyces cerevisiae Sabouraud (Enlarged view). FIG. 7. Saccharomyces cerevisiae on Sabouraud agar. Yeast colonies often resemble bacterial colonies when grown on agar plates. Microscopic examination may be necessary or helpful in identifying colonies as either bacteria or yeast. Here, a colony of common bread yeast, Saccharomyces cerevisiae, was struck for isolated colonies on a plate and incubated at 30°C for 3 days. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 8: Aspergillus niger Sabouraud (Enlarged view). FIG. 8. Aspergillus niger on Sabouraud agar. Aspergillus colonies produce asexual conidiospores by which the organism reproduces itself. These packets of conidiospores can be seen here as the dark "pepper"-appearing grains above the whitish base of the mold colony. This sample was originally collected by opening up a Sabouraud agar plate to the air for 30 minutes, then incubating at room temperature for 5 days. The A. niger conidiospores were then sampled with a sterile loop and patched onto this Sabouraud agar plate, where they grew into colonies after 5 days’ incubation at room temperature. Aspergillus niger is a common environmental contaminant and a fast-growing mold. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 8: Aspergillus niger Sabouraud (Labeled view). FIG. 8. Aspergillus niger on Sabouraud agar. Aspergillus colonies produce asexual conidiospores by which the organism reproduces itself. These packets of conidiospores can be seen here as the dark "pepper"-appearing grains above the whitish base of the mold colony. This sample was originally collected by opening up a Sabouraud agar plate to the air for 30 minutes, then incubating at room temperature for 5 days. The A. niger conidiospores were then sampled with a sterile loop and patched onto this Sabouraud agar plate, where they grew into colonies after 5 days’ incubation at room temperature. Aspergillus niger is a common environmental contaminant and a fast-growing mold. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 9: Two yeasts Sabouraud (Enlarged view). FIG. 9. Two yeasts on Sabouraud agar. Yeast colonies often resemble bacterial colonies when grown on agar plates. These yeasts were obtained from a swab taken from a sink and reisolated from the yeasts grown there by patching two selected colonies on a Sabouraud agar plate and growing at 30°C for 3 days.  This image shows the varying colors and morphologies that different yeast may have. The pinkish-orangish yeast is probably a Rhodotorula species, which are common in the environment. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 10: Penicillium notatum Sabouraud (Enlarged view). FIG. 10. Penicillium notatum on Sabouraud agar. Photograph of the top and reverse of Penicillium notatum growing on Sabouraud agar after 7 days’ growth at room temperature. Note the white "apron" of hyphal growth at the edges of the colonies where spores have not yet developed. The colonies adopt a furrowed appearance (see arrows), also seen on the reverse side, which has developed in the older (i.e., larger) colonies. On the image showing the reverse of the dish, observe the change in coloration: the underside of the colonies is white and/or tan. This is characteristic of Penicillium species. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 10: Penicillium notatum Sabouraud (Labeled view). FIG. 10. Penicillium notatum on Sabouraud agar. Photograph of the top and reverse of Penicillium notatum growing on Sabouraud agar after 7 days’ growth at room temperature. Note the white "apron" of hyphal growth at the edges of the colonies where spores have not yet developed. The colonies adopt a furrowed appearance (see arrows), also seen on the reverse side, which has developed in the older (i.e., larger) colonies. On the image showing the reverse of the dish, observe the change in coloration: the underside of the colonies is white and/or tan. This is characteristic of Penicillium species. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 10: Penicillium notatum Sabouraud (Reverse side). FIG. 10. Penicillium notatum on Sabouraud agar. Photograph of the top and reverse of Penicillium notatum growing on Sabouraud agar after 7 days’ growth at room temperature. Note the white "apron" of hyphal growth at the edges of the colonies where spores have not yet developed. The colonies adopt a furrowed appearance (see arrows), also seen on the reverse side, which has developed in the older (i.e., larger) colonies. On the image showing the reverse of the dish, observe the change in coloration: the underside of the colonies is white and/or tan. This is characteristic of Penicillium species. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 11: Brown exudate Sabouraud (Enlarged view). FIG. 11. Unidentified mold on Sabouraud agar producing an exudate. This mold was isolated from air and grown for 7 days on Sabouraud agar at room temperature. As the mold matured, it began to produce the milky white exudate (arrows). (Janelle Hare, Morehead State University, Morehead, KY)

Figure 11: Brown exudate Sabouraud (Labeled view). FIG. 11. Unidentified mold on Sabouraud agar producing an exudate. This mold was isolated from air and grown for 7 days on Sabouraud agar at room temperature. As the mold matured, it began to produce the milky white exudate (arrows). (Janelle Hare, Morehead State University, Morehead, KY)

Figure 12: Environmental molds Sabouraud (Enlarged view). FIG. 12. Environmental molds on Sabouraud agar at room temperature for 7 days after exposure to air near a household air vent for 30 minutes. Note the variety in size and color, with concentric rings of color developing over time as the colony size increases. Many small colonies are growing on the plate, as a result of sporulation of the parent colonies over time (arrows). On the reverse, note the change in coloration: the underside of these particular mold colonies is yellowish brown. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 12: Environmental molds Sabouraud (Labeled view). FIG. 12. Environmental molds on Sabouraud agar at room temperature for 7 days after exposure to air near a household air vent for 30 minutes. Note the variety in size and color, with concentric rings of color developing over time as the colony size increases. Many small colonies are growing on the plate, as a result of sporulation of the parent colonies over time (arrows). On the reverse, note the change in coloration: the underside of these particular mold colonies is yellowish brown. (Janelle Hare, Morehead State University, Morehead, KY)

Figure 12: Environmental molds Sabouraud (Reverse side). FIG. 12. Environmental molds on Sabouraud agar at room temperature for 7 days after exposure to air near a household air vent for 30 minutes. Note the variety in size and color, with concentric rings of color developing over time as the colony size increases. Many small colonies are growing on the plate, as a result of sporulation of the parent colonies over time (arrows). On the reverse, note the change in coloration: the underside of these particular mold colonies is yellowish brown. (Janelle Hare, Morehead State University, Morehead, KY)