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Author: Multiple Authors​

Citation: Multiple Authors. 2013. Thioglycollate.​

Publication Date: June 2013​

Organisms grown in Fluid Thioglycollate Media​

Organisms grown in Fluid Thioglycollate Media, a reducing media that uses sodium thioglycolate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37° C. Neisseria sicca was grown for 72 hours at 37° C for best results.  Note: Both N. sicca and C. butrycium were grow in supplemented Thioglycollate Media.  A calcium chip can be seen in the bottom of the tube for N. sicca. (A) Escherichia coli:  Facultative Anaerobe, grows both aerobically and anerobically, growth is seen throughout the tube.  Some are capable of growth respiring with oxygen and anaerobically by fermentation (B) Clostridium butrycium: Obligate Anaerobe:  incapable of growth in the presence of oxygen, growth is seen approximatly 1/4 to 1/2 of the way from the top of the tube  (C) Staphylococcus ​aureus: Facultative Anaerobe, grows both aerobically and anerobically, growth is seen throughout the tube  (D) Neisseria sicca: Microaerophile, requires oxygen but at concentrations below atmosphere, grows just below the surface of the media but not at the top  (E) Pseudomonas aeroginosa: Obligate Aerobe:  oxygen is required for growth, growth at the top of the tube only.  Organism will "settle" and sink into the media if longer than 24 hrs. (Tasha Sturm, Cabrillo College, Aptos, CA)​

 

Organisms grown in Fluid Thioglycollate Media​

Organisms grown in Fluid Thioglycollate Media, a reducing media that uses sodium thioglycolate to chemically combine with dissolved oxygen to deplete the oxygen in the media. Organism  were grown for 48 hours at 37o C. (A & C ) Escherichia coli & Staphylococcus​
aureus:  Facultative Anaerobes, grows both aerobically and anerobically, growth is seen throughout the tube compared to  (B) Clostridium butrycium: Obligate Anaerobe: incapable of growth in the presence of oxygen, growth is seen approximatly 1/4 to 1/2 of the way from the top of the tube. (Tasha Sturm, Cabrillo College, Aptos, CA)​

Organisms grown in Fluid Thioglycolate Media​
Neisseria sicca grown in Fluid Thioglycolate Media, a reducing media that uses sodium thioglycolate to chemically combine with dissolved oxygen to deplete the oxygen in the media.   Neisseria sicca was grown for 72 hours at 37° C.   Neisseria sicca: Microaerophile, requires oxygen but at concentrations below atmosphere, Grows just below the surface of the media but not at the top compared to.  Note: for Neisseria sicca supplemented thioglycolate media is used which contains a calcium chip which can be seen in the bottom of the tube.  (Tasha Sturm, Cabrillo College, Aptos, CA)​

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