Download the PowerPoint

PowerPoint Contents

Figure 1: Urease test using urea agar slants. A rapidly positive urease test by Proteus mirabilis (b) is indicated by a color change to bright pink (fuchsia) throughout the urea agar slant as compared to the uninoculated control (a). A delayed positive reaction by Klebsiella pneumoniae is indicated by a color change only along the slant (c).  A negative reaction by Escherichia coli is indicated by the yellow coloration of the media (d). All samples were incubated at 37°C for 16 hours. (Benita A. Brink, Adams State College, Alamosa, CO)

Figure 2: Urease test using urea agar slants. A rapidly positive urease test by Proteus mirabilis (b) is indicated by a color change to bright pink (fuchsia) throughout the urea agar slant as compared to the uninoculated control (a). A delayed positive reaction by Klebsiella pneumoniae is indicated by a color change only along the slant (c).  A negative reaction by Escherichia coli is indicated by the yellow coloration of the media (d). All samples were incubated at 37°C for 16 hours. (Benita A. Brink, Adams State College, Alamosa, CO)

Figure 3: Urease test using urea broth. A positive urease test by Proteus vulgaris (left) is indicated by a color change to bright pink (fuchsia). A negative reaction by Escherichia coli (right) is indicated by the yellow coloration of the media. All samples were incubated at 37°C for 16 hours. (Anne Hanson, University of Maine, Orono, ME)

Figure 4: A positive urease test using urea broth. A positive urease test is indicated by a color change to bright pink (fuchsia). (Diane Hartman, Baylor University, Waco, TX)

Figure 5: A negative urease test using urea broth. A negative urease test is indicated by the yellow coloration of the media. (Diane Hartman, Baylor University, Waco, TX)

Figure 6: Urease test using urea broth. A positive urease test by Proteus (right) is indicated by a color change to bright pink (fuchsia). An uninoculated control shows the yellow-orange color of the uninoculated media. (Clarissa L. Kaup, Bellevue University, Bellevue, NE; J. L. Henriksen, Bellevue University, Bellevue, NE)

Figure 7: Urease test using urea agar slants. A positive urease test by Proteus vulgaris (left) is indicated by a color change to bright pink (fuchsia) throughout the urea agar slant. A negative reaction by Escherichia coli (right) is indicated by the yellow coloration of the media. All samples were incubated at 37°C for 16 hours. (Tasha L Sturm, Cabrillo College, Aptos, CA)

Contact Information

ASM Education, education@asmusa.org