PowerPoint Contents
Figure 1: Infected mouse L cells viewed with bright-field microscopy at 200x magnification. Following virion attachment to cell surface receptors, reovirus particles enter the host cell through receptor-mediated endocytosis. Proteolysis of the outer-capsid proteins occurs via a two-step disassembly process and replication then occurs in the cytoplasm. After assembly of new viral particles, host cells will be lysed to release new infectious viral particles. Maximal viral production within an infected cell occurs about 24 hours postentry.
Figure 2: Infected mouse L cells viewed with epifluorescence at 200x magnification. Cells infected with virus are first tagged with a rabbit anti-reovirus antibody and subsequently tagged with a secondary anti-rabbit antibody, which is fluorescently labeled. A majority of the cells are infected and display a distinct cytoplasmic fluorescence. This image shows the same field of view as Fig. 1.
Figure 3: Mouse L cells infected with a 1/10 viral dilution and viewed with epifluorescence at 200x magnification. The 10-fold viral dilution reduces the amount of infectious virus present, thereby reducing the number of infected mouse L cells and providing a field of view with a countable number of fluorescing cells. Cell density is approximately equal to that of Fig. 1.
Figure 4: Mouse L cells infected with a 1/100 viral dilution and viewed with epifluorescence at 200x magnification. Cell density is approximately equal to that of Fig. 1.
Figure 5: Uninfected mouse L cells viewed with epifluorescence at 200x magnification. Cell density is approximately equal to that of Fig. 1.
Figure 6: Infected mouse L cells infected with a 1/10 viral dilution and viewed with epifluorescence at 200x magnification (Labeled view)
Figure 7: Infected mouse L cells infected with a 1/100 viral dilution and viewed with epifluorescence at 200x magnification (Enlarged view)
Figure 8: Infected mouse L cells infected with a 1/100 viral dilution and viewed with epifluorescence at 200x magnification (Labeled view)
Figure 9: Uninfected mouse L cells viewed with epifluorescence at 200x magnification (Enlarged view)
Figure 10: Uninfected mouse L cells viewed with epifluorescence at 200x magnification (Labeled view)
Contact Information
ASM Education, education@asmusa.org