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This protocol uses EC broth and EC agar media with MUG for E. coli detection in water samples. Conventionally and still practiced in many places, the Standard Analysis of Water, which includes the Most Probable Number (MPN), has to date dominated the scene of coliform and fecal coliform bacterial tests in water samples. The Most Probable Number test is quantitative and can be determined from the result of the presumptive test. EC broth and agar media with MUG are best suited for confirmatory testing of the presence of E. coli after a presumptive positive result for fecal coliform bacteria.

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